High quality Fmoc-Gly-OH Exporter

time：2026-10-22

Fmoc-Gly-OH is a high-purity derivative of glycine with a 9-fluorenylmethyloxycarbonyl (Fmoc) protecting group, designed for researchers and pharmaceutical manufacturers performing solid-phase peptide synthesis (SPPS) who require consistent coupling efficiency and low racemization.

Core Selling Points

Purity ≥ 99% (HPLC) – Minimizes interfering by-products during chain elongation, reducing the need for excessive recrystallization and improving final peptide purity.
Free amine content ≤ 0.1% – Low-level free glycine ensures accurate loading quantification and prevents premature chain termination in automated synthesizers.
Melting point: 172–175 °C – Narrow range provides a reliable identity check and confirms consistent crystal form across lots.
Loss on drying ≤ 0.5% – Low residual moisture contributes to stable weight-based weighing in both manual and automated processes.
Batch-to-batch NMR and HPLC data included – Each lot is shipped with a Certificate of Analysis detailing chromatographic purity, water content, and residual solvents, supporting straightforward documentation in GMP workflows.

Detailed Specification Table

Parameter	Value	Additional Information
Product Name	Fmoc-Gly-OH (Fmoc-Glycine)	CAS No. 29022-11-3
Molecular Formula	C₁₇H₁₅NO₄	Molecular weight: 297.31 g/mol
Appearance	White to off-white crystalline powder	Visual inspection under normal lighting
Purity (HPLC)	≥ 99.0%	Area normalization method, UV detection at 254 nm
Free Amine Content	≤ 0.1%	Determined by TNBS derivatization assay
Melting Point	172–175 °C	Capillary method, uncorrected
Loss on Drying	≤ 0.5%	24 h at 60 °C under vacuum
Solubility	Soluble in DMF, DMSO, THF; sparingly soluble in dichloromethane	Recommended stock concentration: 0.2–0.5 M in DMF for SPPS
Storage Conditions	Store at 2–8 °C, tightly sealed, protected from light	Stable for 24 months from manufacturing date under recommended conditions
Packaging	25 g, 100 g, 500 g, 1 kg	Bulk packaging available on request

Applicable Scenarios

Research laboratories performing manual SPPS – Using Fmoc-Gly-OH for stepwise assembly of glycine-containing peptides (e.g., collagen sequences, amyloid peptides) with reproducible coupling results in standard DIC/HOBt or HATU activation.
Automated peptide synthesizers in discovery settings – The low free-amine content prevents unexpected capping, maintaining accurate step yields for long peptides (20–50 residues).
Process development for generic peptide drugs – The consistent melting point and HPLC profile support scale-up validation studies and facilitate transfer from R&D to pilot plants.
Custom peptide manufacturing (CGMP-like environments) – Full documentation per lot simplifies raw material qualification and audit trails, especially when synthesizing peptides intended for clinical trials.
Educational biochemistry courses – The stable crystalline form is easy to weigh and dissolve, making it suitable for teaching experiments focused on peptide coupling chemistry.

FAQ

What is the CAS number of Fmoc-Gly-OH?
CAS number is 29022-11-3. This is a standard registry number used for chemical purchasing and regulatory documentation.

How do I dissolve Fmoc-Gly-OH for coupling reactions?
Dissolve in DMF at 0.2–0.5 M with gentle heating (max 40 °C) if needed. DMSO is also an option, but DMF is preferred for standard SPPS.

Is Fmoc-Gly-OH stable at room temperature?
Short-term storage (weeks) at room temperature is acceptable, but long-term stability requires 2–8 °C storage away from light and moisture.

Does Fmoc-Gly-OH cause racemization during coupling?
Glycine lacks a chiral center, so racemization is not a concern. The Fmoc group itself is base-sensitive; avoid prolonged exposure to piperidine beyond standard deprotection steps.

What is the typical coupling time for Fmoc-Gly-OH in SPPS?
With DIC/HOBt activation in DMF, 30–60 minutes at room temperature is sufficient. For hindered resins, use HATU with 10–20 minutes and double coupling if needed.

Can Fmoc-Gly-OH be used for solution-phase peptide synthesis?
Yes, it works in solution-phase reactions using standard coupling agents, though solubility in ethyl acetate may be limited; DMF or dichloromethane with a solubilizing co-solvent is recommended.

What purity grade does this product meet?
Minimum HPLC purity is 99.0%, with free amine content below 0.1%. It is not specifically tested for endotoxins, so it is suitable for research and process development, but not for direct use as a sterile pharmaceutical excipient.

Differentiation Summary

Fmoc-Gly-OH from Baishixing offers a combination of high chromatographic purity and tightly controlled free-amine limits that directly affect peptide assembly accuracy. Compared to generic research-grade reagents with purity ranges of 95–98%, this product provides documented lot-specific data for every batch, making it particularly suitable for laboratories and manufacturers that require reproducible synthesis outcomes and traceable quality metrics. It is a practical choice for peptide researchers who prioritize batch consistency over lower-cost alternatives, and it aligns with the needs of preclinical and clinical-stage drug development workflows where raw material variability is a major risk factor.

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