High purity Fmoc-Gly-OH (Fmoc-glycine, CAS 29022-11-3) is a standard Fmoc-protected amino acid derivative used by peptide chemists and pharmaceutical researchers as a building block for solid-phase peptide synthesis (SPPS), enabling the incorporation of glycine residues without undesired side reactions.
| Parameter | Value | Additional Notes |
|---|---|---|
| Product name | Fmoc-Gly-OH (Fmoc-glycine) | Standard Fmoc-protected glycine derivative |
| CAS number | 29022-11-3 | Unique identifier for chemical databases |
| Molecular formula | C₁₇H₁₅NO₄ | Confirmed by elemental analysis |
| Molecular weight | 297.31 g/mol | Monoisotopic mass: 297.100 |
| Purity | ≥ 99% | Determined by HPLC area normalization |
| Appearance | White to off-white powder | Visual inspection under standard lighting |
| Melting point | 175–178 °C | Measured by capillary method |
| Optical rotation | –20.0° to –23.0° (c=1, DMF) | Specific rotation indicates enantiomeric integrity |
| Solubility | Soluble in DMF, DMSO, THF | 50 mg/mL in DMF at 25 °C (typical) |
| Storage conditions | 2–8 °C, light-protected, dry | Sealed container under inert gas |
| Assay (NMR) | Conforms to structure | ¹H NMR and ¹³C NMR spectra provided with COA |
| Heavy metals | ≤ 10 ppm | Meets requirement for research-grade reagents |
| Water content (KF) | ≤ 0.5% | Karl Fischer titration |
What is the CAS number of Fmoc-Gly-OH?The CAS number is 29022-11-3. This registry number is used across chemical databases and safety data sheets to identify the compound in regulatory and purchasing documents.
How should Fmoc-Gly-OH be stored?
Store at 2–8 °C in a tightly sealed container protected from light and moisture. Under these conditions, the product remains stable for up to 24 months from the date of manufacture.
Is Fmoc-Gly-OH soluble in DCM?
Solubility in dichloromethane is limited. It is fully soluble in DMF, DMSO, and THF. For standard SPPS coupling, dissolve in DMF at 0.2–0.5 M concentration.
What is the recommended coupling time for Fmoc-Gly-OH?
Using HBTU/HOBt or DIC/Oxyma activation, complete coupling typically occurs within 30–60 minutes at room temperature. Glycine is less hindered than other amino acids, so reaction times are often shorter.
Can Fmoc-Gly-OH be used in microwave-assisted peptide synthesis?
Yes. The product is compatible with microwave SPPS. Many protocols use 50–70 °C for 5–10 minutes per coupling cycle, but verify with your synthesizer manufacturer's recommendations.
Does high purity affect racemization risk?
High purity and low free amine content reduce the risk of base-mediated racemization during coupling. However, glycine is achiral, so racemization is not a concern for this particular amino acid derivative.
What packing sizes are available from baishixing?
Common package sizes include 25 g, 100 g, 500 g, and 1 kg. Larger custom quantities are available upon request for production-scale synthesis.
Is a certificate of analysis (COA) provided?
Yes. Each batch includes a COA reporting HPLC purity, water content, melting point, and NMR confirmation. Lot-specific documentation enables full traceability.
Compared to standard-grade glycine derivatives, high purity Fmoc-Gly-OH from baishixing offers a standardized purity of ≥ 99% with a documented low water content (≤ 0.5%), which directly impacts the efficiency of coupling reactions in automated peptide synthesizers. Many competing products report purity as ≥ 98%, leaving a wider margin for residual solvents or free Fmoc-glycine. This product’s batch-specific COA and NMR assay provide the traceable quality needed for pharmaceutical R&D and regulated workflows. It is best suited for researchers and manufacturers who require reproducible synthesis outcomes, especially when producing longer peptides (> 20 residues) where even minor impurities can accumulate and complicate purification. For routine, non-critical applications, a lower-grade reagent may be sufficient; however, for final product characterization, process development, or GLP-compliant studies, the consistency of high purity Fmoc-Gly-OH is a practical choice.
