High purity Fmoc-Gly-OH 29022-11-3

Product Overview

Fmoc-Gly-OH (N-alpha-fluorenylmethyloxycarbonyl-glycine) is a high-purity amino acid derivative designed for solid-phase peptide synthesis (SPPS), enabling researchers to achieve efficient coupling reactions with minimal racemization and reduced purification burden.

---

Core Selling Points

Purity ≥ 99.5% (HPLC): Minimizes by-product formation during coupling, reducing final purification steps and improving overall yield.
Appearance — White Crystalline Powder: Consistent, free-flowing powder with clear solubility in common organic solvents (DMF, DCM), ensuring reliable preparation of standard 0.2–0.5 M coupling solutions.
Optical Purity (enantiomeric excess) ≥ 99.9%: Prevents racemization during peptide chain elongation, critical for producing peptides with correct stereochemistry.
Moisture Content ≤ 0.5% (Karl Fischer): Low water content promotes longer shelf life and protects moisture-sensitive coupling reagents like HBTU or HATU.
Batch-to-Batch Consistency: Each batch is validated by HPLC, MS, and ¹H NMR; certificates available to client for direct Lot-to-Lot traceability.

---

Detailed Specifications

Parameter	Value	Remark
Product Name	Fmoc-Gly-OH	N-alpha-Fmoc-L-glycine
CAS Number	29022-11-3	Unique identifier for this amino acid derivative
Molecular Formula	C₁₇H₁₅NO₄	Confirmed by mass spectrometry
Molecular Weight	297.31 g/mol	Calculated
Appearance	White crystalline powder	Visual inspection
Purity (HPLC)	≥ 99.5%	Area normalization method
Optical Purity (e.e.)	≥ 99.9%	Chiral HPLC analysis
Melting Point	174–178 °C	Decomposition point, differential scanning calorimeter
Loss on Drying	≤ 0.5%	Determined by Karl Fischer titration
Solubility	Soluble in DMF, DMSO, DCM; insoluble in water	Tested at 25 °C, 0.5 M in DMF
Residue on Ignition	≤ 0.1%	Heavy metal content control
Storage Conditions	2–8 °C, sealed, dry, dark	Stable for 24 months from production date
Packaging Options	25 g, 100 g, 500 g, 1 kg	Custom bulk packaging available upon request
Regulatory Compliance	ISO 9001:2015 certified manufacturing facility	Quality management system standard

---

Usage Scenarios

Academic Research Laboratories: For researchers synthesizing custom peptides (up to 20 residues) for epitope mapping, enzyme substrate studies, or protein interaction analyses, where consistent coupling efficiency directly dictates experimental reproducibility.
Contract Research Organizations (CROs): For chemists needing a high-purity building block to synthesize peptide libraries for drug screening; high shipment reliability minimizes downtime in automated peptide synthesizers.
Pharmaceutical Peptide Manufacturing: For process developers scaling up GLP/GMP synthesis of peptide APIs, where only a chemically pure and documented amino acid can support process validation and regulatory filings.
Peptide-Based Biomaterials Design: For materials scientists creating self-assembling peptide hydrogels, where low racemization and low impurity profiles prevent uncontrolled gelation behavior.
Educational Advanced Organic Synthesis Courses: For university instructors teaching peptide chemistry modules, allowing students to perform a standard SPPS cycle without complications from moisture or contamination.

---

Frequently Asked Questions

1. What is the shelf life of Fmoc-Gly-OH when stored correctly?Stored at 2–8 °C in the original container, the product retains ≥ 99.5% purity for 24 months. Beyond this, retesting by HPLC is recommended before use.

2. What is the recommended coupling protocol with this product?Use 3.0 equivalents of Fmoc-Gly-OH (0.2–0.5 M in DMF or NMP), activated with HATU/HOBt and DIPEA for standard SPPS. Activation time should not exceed 2 minutes at room temperature to prevent Fmoc deprotection.

3. How can I verify this specific batch meets my specifications?Each batch shipment includes a Certificate of Analysis (CoA) covering all parameters listed in the specifications table. This CoA is also downloadable from the brand's official website using the unique lot number.

4. Is Fmoc-Gly-OH soluble in water?No, it is practically insoluble in water. For aqueous-compatible synthesis, dissolve the amino acid in minimal DMSO or DMF, or use it as a pre-activated ester if required for aqueous conditions.

5. What is the difference between Fmoc-Gly-OH and Boc-Gly-OH?Fmoc chemistry allows orthogonal deprotection using mild bases like piperidine, which is orthogonal to acid-labile side-chain protecting groups. This is the standard for current solid-phase synthesis cycles, unlike Boc chemistry requiring strong acids.

6. Does this product show any batch-to-batch variation in purity?Our established quality protocol maintains variability within 0.2% across production lots. Every batch is tested by HPLC and results are archived for seven years for full traceability.

7. Can this product be used for manufacturing under GMP?Physical (non-GMP) versions are suitable for research development; GMP-compliant batches with additional documentation and validation packages are available as a separate supply chain option.

---

Differentiation Summary

The key distinction of this Fmoc-Gly-OH lies in its combination of high purity (≥ 99.5%) with full analytical documentation (HPLC, MS, ¹H NMR and batch-specific CoA). Unlike competitors that may offer a single summary purity number, this product provides detailed, consistent structural and chromatographic data for every lot, ensuring that qualified, reproducible synthesis is conducted by researchers and manufacturers. The standardized moisture and optical purity controls outperform many standard catalog items that omit these specifics. This version is ideally suited for research teams, CROs, and manufacturing units demanding zero-compromise quality for scale-up procedures or stringent regulatory environment, rather than for steps where cost is the sole criterion and systematic quality checks are not part of the workflow. For direct support on custom specifications or GMP documentation, contact the supplier to request a technical data sheet.