Fmoc-Gly-OH (N-Fmoc-glycine) is a high-purity glycine derivative from Baishixing, intended for labs and production sites that use solid-phase peptide synthesis and need a ≥99% pure building block with traceable batch quality.
Purity ≥99.0% (HPLC)
Reduces the formation of truncated or deletion peptides during coupling, which shortens downstream HPLC purification time for final peptides.
Molecular weight: 297.31 g/mol
Makes reactor loading calculations straightforward; after coupling, glycine contributes a well-defined mass increase of 57.05 Da.
Batch-specific COA with HPLC and LC-MS data
Lets quality teams verify every incoming lot before use, which is important for process reproducibility and development-scale production.
Soluble in DMF and DMSO at room temperature
Works directly with standard SPPS activators such as HATU, DIC, and HOBt in automated synthesizers or manual reactions.
Achiral structure
Glycine has no side chain and no chiral center, so Fmoc-Gly-OH does not racemize; this simplifies chiral purity control in peptide intermediates.
| Parameter | Value | Remarks |
|---|---|---|
| Product name | N-Fmoc-glycine | Also written as Fmoc-Gly-OH |
| Chemical formula | C₁₇H₁₅NO₄ | Urethane-protected glycine |
| Molecular weight | 297.31 g/mol | For stoichiometric calculations |
| CAS number | 29022-11-3 | Registry number for ordering |
| Purity (HPLC) | ≥99.0% | Measured area normalization; exact value on batch COA |
| Appearance | White crystalline powder | Standard form for SPPS reagents |
| Melting point | 174–175 °C | Literature range; actual value on COA |
| Optical rotation | Not applicable | Glycine is achiral |
| Solubility | Soluble in DMF and DMSO; sparingly soluble in water | No heating required for standard peptide coupling |
| Recommended storage | −20 °C, sealed, protected from light and moisture | Avoid repeated freeze-thaw cycles |
| QC data | HPLC, LC-MS, loss on drying | Shipped with lot-specific certificate of analysis |
Automated SPPS of therapeutic peptides
Peptide chemists can use Fmoc-Gly-OH directly in standard coupling cycles; the high purity lowers failure sequences and improves crude peptide quality.
Scale-up of peptide intermediates
Process chemists who move from gram-scale to multi-kilogram batches can keep equivalent stoichiometry because each lot has consistent HPLC purity and documented identity.
Peptide library synthesis
Medicinal chemistry teams generating glycine-containing libraries get uniform coupling behavior across wells, making screening results easier to compare.
Diagnostic peptide conjugates
Researchers preparing glycine-linked conjugates observe fewer side-product peaks in LC-MS, which simplifies purification and conjugation ratio control.
HPLC method development
Because Fmoc-Gly-OH has a defined molecular weight and no chiral center, it is a practical reference material for column calibration and system suitability tests.
What is the CAS number for Fmoc-Gly-OH?
The CAS number is 29022-11-3. This registry number identifies N-Fmoc-glycine and is used for procurement, inventory records, and safety documentation. Confirming the CAS number before ordering prevents mix-ups with other glycine derivatives.
Why does Fmoc-Gly-OH purity matter in peptide synthesis?
Residual glycine or Fmoc-degradation by-products can compete in the coupling step and generate shortmer impurities. These make preparative chromatography harder and reduce final peptide purity. Using ≥99.0% HPLC material keeps coupling stoichiometry accurate and reduces purification cycles.
How should Fmoc-Gly-OH be stored?
Store at −20 °C in a tightly sealed container, protected from light and moisture. Let the bottle warm to room temperature before opening to avoid water condensation. Under these conditions, the material remains suitable for SPPS for long-term storage.
Can Fmoc-Gly-OH be used in automated peptide synthesizers?
Yes. It dissolves in DMF and DMSO near room temperature, so it can be dispensed by standard automated synthesizers. For best results, prepare fresh solutions and avoid prolonged exposure to bases that could remove the Fmoc group prematurely.
Is Fmoc-Gly-OH optically active?
No. Glycine is achiral, so Fmoc-Gly-OH has no optical rotation. This makes it useful as a non-chiral reference material and as a positive control when optimizing chiral HPLC methods for other Fmoc-amino acids.
What is the difference between Fmoc-Gly-OH and Boc-Gly-OH?
Fmoc-Gly-OH uses a base-labile Fmoc protecting group; Boc-Gly-OH uses an acid-labile Boc group. Fmoc chemistry matches automated SPPS protocols with mild deprotection, while Boc chemistry requires strong acid cleavage after each cycle.
For labs that need a glycine building block for research, QC, or scale-up, the Fmoc-Gly-OH supplied by Baishixing provides a cleaner starting point than uncharacterized catalog reagents: each batch is tested by HPLC and LC-MS, and the certificate of analysis travels with the order. It is not a universal fix for poor coupling conditions, but in a well-controlled SPPS workflow it removes one controllable variable. Laboratories working with glycine-rich peptides, automated synthesizers, or method validation will gain the most from this product.
