High purity Fmoc-Gly-OH Manufacturer

Fmoc-Gly-OH (Fmoc-glycine) is a Fmoc-protected amino acid derivative used as a building block in solid-phase peptide synthesis, enabling accurate glycine incorporation with minimal side reactions for research and manufacturing applications.

Core Selling Points

Purity ≥ 99.0% (HPLC): Reduces deletion sequences and re-purification steps, improving final peptide yield in challenging syntheses.
Melting point 85.0–90.0 °C: Consistent thermal profile supports reproducible coupling reactivity across different batches.
Water content ≤ 0.1% (Karl Fischer): Prevents hydrolysis of active ester species, extending coupling efficiency in automated synthesizers.
Solubility ≥ 100 mg/mL in DMF at 25 °C: Enables flexible concentration adjustments for both standard and high-load synthesis protocols.
Steady supply from 10 g to multi-kilogram quantities: Backed by baishixing’s dedicated production line, ensuring reliable lead times for scale-up projects.

Detailed Parameter Table

Parameter	Value	Supplementary Notes
CAS Number	29022-11-5	Verified against SciFinder reference
Molecular Formula	C₁₇H₁₅NO₄	Consistent with Fmoc-glycine structure
Molecular Weight	297.31 g/mol	Average mass as per IUPAC standard
Appearance	White to off-white powder	Visual inspection under 1x magnification
Purity (HPLC)	≥ 99.0%	Area normalization method, UV detection at 254 nm
Melting Point	85.0–90.0 °C	Determined by capillary melting point apparatus
Water Content	≤ 0.1%	Measured via Karl Fischer titration
Solubility in DMF	≥ 100 mg/mL at 25 °C	Clear colorless solution after 5 min stirring
Storage Conditions	Sealed, dry, −20 °C to 8 °C	Protect from light and moisture
Shelf Life	24 months	Retains ≥ 99% purity when stored accordingly

Applicable Scenarios

Academic peptide research: A graduate scientist conducting structure-activity relationship studies uses Fmoc-Gly-OH to introduce glycine residues at multiple positions, achieving reliable coupling rates in 3,000–5,000 molecular weight peptides.
Pharmaceutical lead optimization: A medicinal chemist preparing 10–50-mer peptide analogs relies on consistent batch-to-batch purity to avoid false biological activity results caused by truncated impurities.
Peptide drug GLP production: A process chemist scale-up from 100 g to 2 kg needs low water content and high solubility to maintain homogeneous reaction mixtures in large reactors.
Contract synthesis services: A CRO handling diverse client sequences appreciates rapid dissolution time and uniform quality for automated parallel synthesizers running 24-well plates.
Biotechnology kit development: A product developer embedding peptides in cell-culture substrates selects this reagent for its stable coupling profile, ensuring reproducible peptide loading across multiple production lots.

Frequently Asked Questions

What is the CAS number of Fmoc-Gly-OH?
29022-11-5. This is the official Chemical Abstracts Service registry number for N-Fmoc-glycine.

How should Fmoc-Gly-OH be stored after opening?
Store the bottle tightly sealed in an anhydrous desiccator at −20 °C to 8 °C, and allow it to equilibrate to room temperature before opening to avoid moisture condensation.

What is the typical coupling time for this product in SPPS?
In standard Fmoc solid-phase synthesis with HBTU activation, coupling reaches > 99% within 30 minutes at room temperature using 3-fold excess of amino acid, though double coupling is recommended for difficult sequences.

Can I use Fmoc-Gly-OH in flow-based peptide synthesizers?
Yes. Its high solubility in DMF and DMA (≥ 100 mg/mL) supports the rapid flow mixing used in flow chemistry platforms without clogging lines.

What impurities are specified beyond the 99% HPLC purity?
Residual solvents (ethanol ≤ 0.5%), acetic acid ≤ 0.1%, and free glycine ≤ 0.1% are controlled per product specification sheet.

Does baishixing provide analytical documentation with each shipment?
Each batch includes a Certificate of Analysis covering HPLC purity, melting point, water content, and a ¹H-NMR spectrum, compliant with ISO 9001 quality management traceability.

Is this product suitable for GMP manufacturing of peptide drugs?
It is manufactured in a GMP-compliant facility with full batch records and impurity profiling, yet users should perform their own quality system evaluation, as no raw material alone guarantees final drug approval.

How does this Fmoc-Gly-OH compare to glycine ethyl ester derivatives?
Fmoc-Gly-OH carries a base-labile Fmoc group directly applicable to standard Fmoc/tBu SPPS, whereas ethyl esters require additional saponification steps, making this product more operationally straightforward for peptide assembly.

Differentiation Summary

Unlike general-purity Fmoc-glycine available from fine chemical suppliers, baishixing Fmoc-Gly-OH delivers a narrowed purity window (≥ 99.0% with controlled free glycine and solvent levels) that directly addresses the reproducibility demands of peptide research scale-up. Its combination of low water content, high DMF solubility, and comprehensive batch documentation offers a practical advantage for laboratories transitioning from qualitative discovery to production-grade peptide synthesis. For researchers and process chemists who prioritize consistent coupling behavior and supply reliability without paying a premium for consumables branding, this product provides a balanced choice combining material performance with industrial scalability.