Fmoc-Gly-OH 29022-11-5 Exporter

Fmoc-Gly-OH is an N-Fmoc–protected glycine building block for solid-phase peptide synthesis (SPPS), manufactured at ≥99% HPLC purity to improve coupling yields and minimize side reactions in both research and GMP-compliant peptide production.

Key Selling Points

Purity ≥99% (HPLC): Each batch is tested at 220 nm and 254 nm, ensuring predictable coupling kinetics and reduced accumulation of deletion-related impurities across long peptide sequences.  
Low free glycine content: The FK-102 impurity is controlled to ≤0.1%, preventing premature chain termination and lowering the burden on preparative HPLC purification.  
Documented batch traceability: A certificate of analysis (CoA) is supplied for every lot, covering purity, water content, melting point, and residual solvents according to ICH Q3C(R8) and EP current monographs.  
36-month shelf life at 2–8 °C: Real-time stability data confirm the product remains within release specifications for three years if stored away from moisture and light.  
Flexible supply scale: Packaging ranges from 100 g research bottles to 25 kg drums, allowing a seamless transfer from discovery to clinical-stage manufacturing without changing raw material specifications.

Detailed Specifications

Parameter	Value	Supplementary Information
Product Name	Fmoc-Gly-OH	Also known as N-Fmoc-glycine or N-(9-fluorenylmethoxycarbonyl)glycine
IUPAC Name	2-({[(9H-fluoren-9-yl)methoxy]carbonyl}amino)acetic acid	Systematic chemical name
CAS Number	29022-11-3	Unique registry number
Molecular Formula	C₁₇H₁₅NO₄	Consistent with monoisotopic mass 297.10 Da
Molecular Weight	297.31 g/mol	Average molar mass
Appearance	White to off-white powder	Visual inspection per SOP
Purity (HPLC)	≥99.0%	Area normalization at 220 nm and 254 nm
Free Glycine	≤0.1%	Determined by HPLC after post-column derivatization
Melting Point	174–178 °C	Differential scanning calorimetry
Loss on Drying	≤0.5%	Gravimetric at 105 °C for 3 h
Heavy Metals	≤20 ppm	ICP-MS analysis per EP 2.5.14
Residual Solvents	Meets ICH Q3C(R8)	Class 2 and Class 3 solvents are monitored
Storage Temperature	2–8 °C	Keep container tightly closed and protected from light
Shelf Life	36 months	Verified with real-time stability studies
Packaging Options	100 g, 1 kg, 5 kg, 25 kg	Custom bulk sizes available by request

Applicable Scenarios

Solid-phase peptide synthesis (SPPS): Use as the C-terminal or internal amino acid; the high purity supports efficient chain elongation without detection of repetitive loss of Fmoc protection.  
Peptide drug intermediate manufacturing: Low heavy metal and residual solvent levels align with GMP requirements for clinical-stage peptide APIs, including investigational new drug (IND) studies.  
Fragment condensation synthesis: Reliable coupling efficiency enables assembly of protected peptide fragments for convergent strategies, especially in the 10–50 mmol scale range.  
Automated high-throughput peptide synthesis: The free-flowing powder dissolves rapidly in DMF or NMP, ensuring uniform resin loading and consistent liquid transfer in 384-well parallel synthesizers.  
Analytical method development and quality control: This material can serve as a standard reference for quantifying Fmoc-glycine content and for validating chromatographic methods intended for peptide purity testing.

FAQ

What is Fmoc-Gly-OH used for?
It is used as a protected glycine monomer in SPPS. The Fmoc group is removed with piperidine under mild conditions, enabling stepwise assembly of peptides from the C-terminal to the N-terminal direction.

What is the CAS number of Fmoc-Gly-OH?
The CAS registry number is 29022-11-3. This identifier is widely recognized in chemical catalogs, regulatory filings, and safety data sheets.

How should Fmoc-Gly-OH be stored?
Store at 2–8 °C in a tightly closed container, protected from moisture and light. Under these conditions, the product retains its specifications for 36 months.

What purity is guaranteed?
Each lot is released at ≥99.0% purity measured by HPLC area normalization at two UV wavelengths. The exact chromatographic profile is included in the certificate of analysis.

Is Fmoc-Gly-OH compatible with automated peptide synthesizers?
Yes. It dissolves readily in DMF and NMP, and its low hygroscopicity prevents nozzle clogging in automated fluidics systems.

What is the difference between Fmoc-Gly-OH and Boc-Gly-OH?
Fmoc-Gly-OH uses a base-labile Fmoc group for orthogonal protection, while Boc-Gly-OH uses an acid-labile Boc group. Fmoc chemistry is more common in modern SPPS because deprotection is milder.

Can I obtain a certificate of analysis before ordering?
Yes. A representative CoA is available on request, and lot-specific CoAs accompany every shipment. You can request the instrument conditions for HPLC purity and residual solvent tests.

Does Fmoc-Gly-OH have any chiral center?
No. Glycine is achiral, so Fmoc-Gly-OH does not require chiral purity testing. This simplifies quality control compared with Fmoc-alanine or other chiral amino acids.

Distinctive Advantage Summary

Compared with general-purpose Fmoc-glycine reagents, the product supplied through the Baishixing quality system provides a clear advantage in pharmacopeial-grade purity documentation and lot-to-lot consistency. Every batch is released with detailed analytical data covering free glycine, residual solvents, and trace metals, which allows peptide chemists to adopt the material without performing extensive re-validation. The real cost benefit appears when failed coupling reactions and purification rework are reduced. This reagent is especially suitable for early-phase drug development, GMP manufacturing, and academic laboratories that require reproducible SPPS performance across different researchers and instruments.