High Purity Fmoc-Gly-OH is an Fmoc-protected glycine derivative (CAS 29022-11-3) for solid-phase peptide synthesis, used by research chemists and process development teams to reduce by-product formation, avoid chain termination, and simplify downstream purification.
| Parameter | Value | Supplementary Note |
|---|---|---|
| Product Name | High Purity Fmoc-Gly-OH | Also known as Fmoc-glycine |
| CAS Number | 29022-11-3 | Unique chemical registry identifier |
| Molecular Formula | C₁₇H₁₅NO₄ | Fmoc group attached to glycine |
| Molecular Weight | 297.31 g/mol | Calculated from atomic weights |
| Appearance | White crystalline powder | Visual inspection |
| Purity (HPLC) | ≥99.0% | Area normalization method |
| Free Glycine | ≤0.1% | Cation-exchange HPLC |
| Chloride | ≤100 ppm | Ion chromatography |
| Acetate | ≤100 ppm | Ion chromatography |
| Water Content | ≤0.5% | Karl Fischer titration |
| Solubility | ≥50 mg/mL in DMF | Clear solution at 25 °C |
| Storage | −20 °C, sealed, dry | Stable for 24 months from manufacture date |
| Packaging | Inert, moisture-protected container | Available in multiple pack sizes |
Specifications above are based on the manufacturer’s Certificate of Analysis for this product line. Exact values should be verified with the lot-specific COA.
What is Fmoc-Gly-OH used for?
Fmoc-Gly-OH is a protected glycine building block in solid-phase peptide synthesis. The Fmoc group protects the amine during coupling. After coupling, piperidine removes the Fmoc group, exposing the amine for the next amino acid addition.
What purity should I expect from “high purity” Fmoc-Gly-OH?
High purity means ≥99.0% HPLC purity with controlled levels of free glycine, chloride, acetate, and water. For this product, free glycine is ≤0.1%, chloride and acetate are each ≤100 ppm, and water content is ≤0.5%.
How should Fmoc-Gly-OH be stored?
Store at −20 °C in a sealed, dry container, protected from light and moisture. Before opening, allow the container to reach room temperature to prevent water condensation. Under these conditions, shelf life is 24 months from the manufacture date.
How does Fmoc-Gly-OH compare with Boc-Gly-OH?
Fmoc-Gly-OH uses a base-labile Fmoc group and fits Fmoc/tBu SPPS protocols. Boc-Gly-OH requires acidic deprotection and is used in Boc/benzyl strategies. For automated peptide synthesizers and milder synthesis conditions, Fmoc-Gly-OH is the more practical choice.
Does high purity prevent racemization of glycine?
Glycine does not have a chiral center, so it does not racemize like alanine or phenylalanine derivatives. However, high purity reduces related side reactions such as diketopiperazine formation and chain termination, which improves overall coupling fidelity.
Can I use high purity Fmoc-Gly-OH in an automated peptide synthesizer?
Yes. Its low free-glycine content and consistent solubility in DMF at 50 mg/mL allow reliable dispensing and coupling. It works with standard Fmoc SPPS protocols using HBTU, HATU, DIC, or HOBt activation.
What analytical method is used to verify purity?
Purity is measured by HPLC with UV detection at 254 nm or 210 nm. The COA reports area-normalized purity, related-substance peaks, and residual ion content by ion chromatography. Each batch is issued with its own COA.
Compared with generic Fmoc-Gly-OH materials that only state a single HPLC purity number, this product specifies free glycine, chloride, acetate, and water content on every lot. The batch-specific COA gives peptide chemists a clear picture of what enters the synthesis column, which supports regulatory-facing research and easier process transfer. This grade is suitable for users who need consistent coupling performance, low purification overhead, and traceable raw material documentation. High Purity Fmoc-Gly-OH supplied by Baishixing is a practical choice for laboratories that treat amino acid quality as part of the synthesis result.
