High quality Fmoc-Gly-OH 29022-11-5

1. Product Definition

Fmoc-Gly-OH (Fmoc-Glycine) is an Fmoc-protected glycine derivative for solid-phase peptide synthesis (SPPS), designed for peptide chemists and process developers who require a consistent, high-purity building block to reduce side reactions and improve coupling reliability.

2. Core Features and Benefits

≥99.0% HPLC purity – Reduces the risk of racemization and by-product formation, simplifying final peptide purification and improving overall yield.
≤0.1% free amine content – Prevents chain termination and deletion sequences during peptide elongation, especially in long or difficult syntheses.
≤0.5% water content (Karl Fischer) – Ensures stable solubility and predictable coupling kinetics in standard SPPS solvents such as DMF and NMP.
Batch-specific COA with full analytical data – Allows systematic quality verification for GMP-aligned processes and reproducible research outcomes.
Scale-up packaging from 25 g to 100 kg – Maintains the same impurity specifications from lab-scale experiments to commercial manufacturing.

3. Technical Specifications

Parameter	Value	Additional Notes
Chemical Name	Fmoc-Gly-OH (Fmoc-glycine)	N-(9-Fluorenylmethoxycarbonyl)glycine
CAS Number	29022-11-3	Unique identifier used in databases and procurement
Molecular Formula	C17H15NO4	Confirmed by mass spectrometry
Molecular Weight	297.31 g/mol	Used for molar equivalent calculations
Purity (HPLC)	≥99.0%	Determined by RP-HPLC at 210 nm, per lot COA
Free Amine (as glycine)	≤0.1%	Verified by TLC / derivatization method
Water Content (KF)	≤0.5%	Measured by Karl Fischer titration
Appearance	White to off-white crystalline powder	Visual inspection per QC protocol
Solubility	Freely soluble in DMF and DMSO; soluble in THF and acetonitrile	100 mg/mL in DMF at 20 °C
Melting Point	174–176 °C (dec.)	Decomposition observed at melting
Storage Conditions	2–8 °C, protected from light and moisture	Stable for at least 24 months under recommended conditions
Quality Management	ISO 9001:2015 certified production facility	Certificate available on request with COA

4. Practical Application Scenarios

Academic peptide labs – Researchers using manual SPPS can introduce glycine residues at internal or C-terminal positions with minimal failure sequences because of the low free-amine content.
CRO peptide synthesis services – Contract research organizations processing multiple custom peptides benefit from batch-to-batch consistency, reducing re-synthesis and re-purification cycles.
Pharmaceutical process development – Process chemists scaling up peptide APIs need documented impurity profiles; the provided COA supports process validation and regulatory documentation.
High-throughput peptide libraries – Screening groups producing libraries on automated synthesizers rely on low moisture and high solubility to avoid clogging and variable coupling efficiency.
Bioconjugation and ADC linker synthesis – Researchers making peptide linkers use Fmoc-Gly-OH as a spacer to ensure precise chain elongation and minimize linker-related impurities.

5. Frequently Asked Questions

1. What is the CAS number of Fmoc-Gly-OH?
The CAS number for Fmoc-Gly-OH is 29022-11-3. This unique numerical identifier is assigned to Fmoc-protected glycine and is widely used in chemical databases, safety data sheets, and procurement documents to avoid ambiguity. Always reference this CAS number when ordering.

2. What purity is available for Fmoc-Gly-OH?
Fmoc-Gly-OH is supplied with a purity of ≥99.0% as determined by HPLC. Each batch is tested and the chromatogram is included in the certificate of analysis to confirm the actual purity value, providing traceable quality data for R&D and manufacturing processes.

3. How should Fmoc-Gly-OH be stored?
Store Fmoc-Gly-OH at 2–8 °C in a tightly sealed container, protected from light and moisture. Under these conditions, the product maintains specification for at least 24 months from the date of manufacture, as verified by stability studies.

4. Is Fmoc-Gly-OH suitable for automated peptide synthesizers?
Yes. Its low water content and high solubility in DMF make it compatible with common automated SPPS protocols. It can be dissolved at 100 mg/mL in DMF without residue or gel formation, allowing precise delivery during coupling cycles on automated synthesizers.

5. What are the main impurities in Fmoc-Gly-OH?
Typical impurities include free glycine, Fmoc-Gly-Gly, and residual solvents. High-quality material limits free glycine to ≤0.1% and total related substances to under 1%, as verified by HPLC and TLC, which minimizes side reactions in peptide synthesis.

6. Does Fmoc-Gly-OH require special handling precautions?
No special hazards are reported for standard laboratory use. Handle in a well-ventilated area with standard protective gloves and safety glasses. Avoid prolonged exposure to strong acids or bases because the Fmoc group is base-labile and may be cleaved under basic conditions.

7. Can Fmoc-Gly-OH be used as a starting material for Fmoc-glycine derivatives?
Yes. The carboxylic acid group can be activated to form esters, amides, or acid chlorides for further derivatization. The Fmoc group remains stable during these transformations when conducted under mild, non-basic conditions at temperatures below 25 °C.

8. What is the molecular weight of Fmoc-Gly-OH?
The molecular weight is 297.31 g/mol. This value is confirmed from the molecular formula C17H15NO4 and is used for calculating molar equivalents in peptide coupling reactions, resin loading, and yield estimation. Always use the exact value when preparing stock solutions.

6. Differentiation Summary

Fmoc-Gly-OH is a standard reagent, but its utility depends on the consistency of key impurities. The baishixing manufacturing process for this product specifically controls free amine and water contents to tight thresholds, while each lot is released with a COA that includes HPLC, Karl Fischer, and residual-solvent data. This approach distinguishes it from generic laboratory-grade amino acids that may vary batch to batch and cause unpredictable peptide synthesis results. It is best suited for researchers and production teams who require documented quality, process reproducibility, and a supply source that can scale from milligrams to metric tons.