High purity Fmoc-Gly-OH Factory

High purity Fmoc-Gly-OH is a fluorenylmethyloxycarbonyl-protected glycine derivative from Baishixing, designed for researchers and peptide manufacturers who need a reliable building block for solid-phase peptide synthesis with minimal impurities and reproducible coupling results.

Core Selling Points

Purity ≥ 99.0% by HPLC – Ensures consistent coupling efficiency, reduces the need for re-purification, and minimizes by-product formation in peptide chains.
Low racemization rate (<0.1%)– Maintains optical integrity during synthesis, which is critical for peptides that require exact stereochemistry (e.g., therapeutic peptides).
Batch-to-batch consistency – Each batch is verified with a Certificate of Analysis (COA) including specific rotation, water content (≤ 0.5%), and residual solvent data, allowing easier validation in GMP workflows.
High solubility in standard coupling solvents – Fully dissolves in DMF, DCM, and NMP at concentrations up to 200 mg/mL at 25°C, enabling efficient activation without extra heating or sonication.
Stable under standard storage conditions – When kept sealed at 2–8°C away from light, the product maintains ≥ 99% purity for 36 months, as stated in the manufacturer’s stability study.

Detailed Parameter Table

Parameter	Value	Supplementary Notes
CAS Number	29022-11-3	Assigned by Chemical Abstracts Service
Molecular Formula	C₁₇H₁₅NO₄	Confirmed by elemental analysis (C: 68.68%, H: 5.08%, N: 4.71%)
Molecular Weight	297.31 g/mol	Calculated using monoisotopic masses
Appearance	White to off-white crystalline powder	Visual check against reference standard
Purity (HPLC)	≥ 99.0%	Area percent, C18 reversed-phase column, UV detection at 220 nm
Water Content (KF)	≤ 0.5%	Karl Fischer titration, ensures dry handling
Melting Point	174–176°C	Determined by differential scanning calorimetry (DSC)
Specific Rotation [α]₂₀ᴰ	-15.5° ± 1.0° (c=1, DMF)	Measured on a polarimeter at 20°C, D-line
Solubility in DMF	≥ 200 mg/mL at 25°C	Measured by UV-Vis saturation method
Storage Temperature	2–8°C	Sealed, dry, and protected from light
Shelf Life	36 months	Under recommended conditions, verified by stability batches

Applicable Scenarios

Solid-phase peptide synthesis (SPPS) – Used as the C-terminal glycine building block for assembling peptides on resin; high purity reduces deletion sequences and simplifies HPLC purification of the final peptide.
Peptide drug intermediate production – Suitable for manufacturing of peptide APIs where racemization and impurity profiles directly affect regulatory compliance; the low water content helps stability in large-scale coupling reactions.
Research on peptide conjugates – Researchers modifying glycine residues with fluorescent labels or PEG chains can rely on the consistent reactivity of Fmoc-Gly-OH without unexpected side reactions.
Custom peptide libraries – When producing multiple analogues, the batch consistency allows for comparative structure-activity studies without introducing variables from different starting material quality.
Clinical trial supply manufacturing – For cGMP environments, the provided COA, traceability documents, and stability data support documentation requirements and reduce vendor qualification time.

Common Questions

What is the difference between Fmoc-Gly-OH and Boc-Gly-OH?
Fmoc-Gly-OH uses a base-labile Fmoc group that is removed with piperidine, while Boc-Gly-OH uses an acid-labile Boc group. Fmoc chemistry is more common in automated SPPS due to milder deprotection conditions.

How do I verify the purity of Fmoc-Gly-OH before use?
Run HPLC using a C18 column with a gradient of acetonitrile/water containing 0.1% TFA. Compare retention time and area percent to the supplier’s COA. Typical retention time for Fmoc-Gly-OH is 9–11 minutes under standard conditions.

Can Fmoc-Gly-OH be used in flow peptide synthesis?
Yes. Its high solubility in DMF and NMP allows rapid mixing and activation at room temperature, making it compatible with flow-based synthesizers where contact times are short.

How should Fmoc-Gly-OH be stored to maintain its quality?
Store it in a tightly sealed, light-resistant container at 2–8°C. Keep it away from acids, bases, and oxidizing agents. Allow the container to reach room temperature before opening to prevent moisture condensation.

Is racemization a concern with Fmoc-Gly-OH?
Glycine has no chiral center; thus racemization of the amino acid itself is not possible. However, coupling activators can cause epimerization of the adjacent amino acid. Using HATU or DIC/Oxyma with Fmoc-Gly-OH at 0°C reduces this risk.

What is the recommended coupling protocol for Fmoc-Gly-OH?
Use 3-fold excess of Fmoc-Gly-OH with HBTU/HOBt or HATU in DMF, with 6 equivalents of DIPEA for 30–60 minutes at room temperature. After coupling, wash extensively with DMF to remove excess reagent.

Does high purity affect the cost per synthesis cycle?
While the initial cost per gram may be slightly higher than lower-purity grades, the reduction in failed couplings and purification steps often lowers the overall cost per purified peptide. This is especially noticeable in long peptides (>30 residues).

Differentiation Summary

Compared to standard peptide-grade Fmoc-Gly-OH from other vendors, the Baishixing high-purity grade offers a tightened specification for water content (≤0.5%), a documented specific rotation range, and a 36-month stability claim backed by real-time data. These features are particularly valuable for contract manufacturing organizations (CMOs) and R&D labs that must demonstrate reliable raw material quality during audits or when troubleshooting low-yield syntheses. While any high-purity Fmoc-Gly-OH will perform adequately in routine SPPS, this product stands out for its batch-to-batch documentation and suitability for peptides intended for in vivo studies, where trace impurities could trigger unwanted immune responses or toxicity. Choose this grade if you prioritize process reproducibility, regulatory readiness, and minimal interference from residual water or reactive impurities.