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Fmoc-Gly-OH (N-Fmoc-glycine, CAS 29022-11-3) is a high-purity amino acid derivative used as a key building block in solid-phase peptide synthesis, providing researchers and pharmaceutical manufacturers with a reliable reagent for coupling glycine residues with minimal side reactions and reproducible coupling efficiency.

Core Selling Points

Purity ≥99% (HPLC) – Ensures low byproduct formation and consistent chain assembly, reducing the need for costly purification steps.
Free glycine content ≤0.3% – Minimizes premature chain termination and racemization during peptide elongation, improving overall yield.
Batch-to-batch consistency – Each lot is supplied with a Certificate of Analysis (COA), allowing users to verify identity and purity before use.
Stable crystalline powder – Store at 2–8 °C under light protection; the material remains chemically stable for over 24 months when unopened.
Wide solvent compatibility – Fully soluble in DMF, DCM, THF, and NMP, facilitating its use in both automated and manual synthesis protocols.

Detailed Specification Table

Parameter	Value	Supplementary Notes
Product Name	Fmoc-Gly-OH	IUPAC: (9H-fluoren-9-yl)methyl (glycyl)carbamate
CAS Number	29022-11-3	Unique identifier for regulatory and ordering purposes
Molecular Formula	C17H15NO4	Includes the fluorenylmethyloxycarbonyl protecting group
Molecular Weight	297.31 g/mol	Calculated from the molecular formula
Purity (HPLC)	≥99.0%	Determined by high-performance liquid chromatography
Appearance	White to off-white crystalline powder	No visible particulates or discoloration
Melting Point	172–176 °C	Characteristic range for Fmoc-Gly-OH
Free Glycine	≤0.3%	Determined by HPLC; indicates residual unprotected glycine
Water Content	≤0.5%	Measured by Karl Fischer titration
Solubility	Soluble in DMF, DCM, THF, NMP (≥0.1 g/mL)	Clear solution without precipitate
Storage Conditions	2–8 °C, protected from light and moisture	Keep container tightly sealed after opening
Shelf Life	24 months from date of manufacture	When stored under recommended conditions

Applicable Scenarios

Academic Research: Use Fmoc-Gly-OH to synthesize custom peptides for epitope mapping, enabling precise identification of antibody binding sites.
Pharmaceutical R&D: Incorporate this reagent into solid-phase syntheses of peptide drug candidates, ensuring high coupling efficiency during lead optimization.
Contract Manufacturing: Utilize this building block in large-scale peptide production (up to kilogram quantities) where lot-to-lot reproducibility is critical for GMP compliance.
Peptide Library Synthesis: In automated parallel synthesizers, Fmoc-Gly-OH provides the necessary solubility and purity to generate diverse peptide libraries for high-throughput screening.
Basic Biochemical Studies: Researchers investigating peptide-protein interactions can rely on this reagent to prepare stable glycine-containing peptides with defined sequences.

Common Questions and Answers

Q1: What is Fmoc-Gly-OH used for?
Fmoc-Gly-OH is an amino acid building block in solid-phase peptide synthesis. It introduces glycine residues while the Fmoc group protects the amine, allowing controlled chain assembly. This reagent is essential for producing both short peptides and larger peptide-based drugs, making it a staple in peptide chemistry.

Q2: How should Fmoc-Gly-OH be stored?
Store Fmoc-Gly-OH in a tightly sealed container at 2–8 °C, protected from direct light and humidity. Avoid repeated opening of the container to minimize moisture absorption. Under these conditions, the powder remains stable for up to 24 months without significant degradation. Always check the COA for specific storage recommendations.

Q3: What purity can I expect from Fmoc-Gly-OH?
The standard product purity is ≥99% as determined by HPLC. In addition, free glycine is controlled to ≤0.3%, and water content to ≤0.5%. These limits ensure minimal interference during coupling reactions, resulting in cleaner peptide products. Each batch’s COA confirms the measured values.

Q4: Can Fmoc-Gly-OH be used in automated peptide synthesizers?
Yes, Fmoc-Gly-OH is fully compatible with automated solid-phase synthesizers. Its high purity, consistent particle size, and good solubility in common solvents like DMF make it suitable for both small-scale research instruments and production-scale systems. This versatility enables seamless transfer from R&D to manufacturing.

Q5: What is the difference between Fmoc-Gly-OH and Boc-Gly-OH?
Fmoc-Gly-OH uses a base-labile fluorenylmethoxycarbonyl (Fmoc) protecting group, which is removed with a secondary amine such as piperidine. Boc-Gly-OH uses an acid-labile tert-butyloxycarbonyl (Boc) group, requiring strong acid for deprotection. The choice depends on the overall synthesis strategy. Both methods are widely used, but Fmoc chemistry is more common in modern SPPS.

Q6: Is Fmoc-Gly-OH suitable for large-scale production?
Yes, when supplied with the appropriate documentation, Fmoc-Gly-OH can be used in large-scale peptide manufacturing. It is essential to verify batch-specific COA values and ensure that the material meets required specifications. Many contract manufacturers use this product in multi-kilogram batches.

Q7: Which impurities are typically monitored in Fmoc-Gly-OH?
Common controlled impurities include free glycine, water, residual solvents (e.g., ethyl acetate, acetonitrile), and related Fmoc compounds. Each batch is analyzed by HPLC and Karl Fischer titration to ensure these impurities stay within the limits specified on the certificate of analysis.

What Makes This Product Different?

Compared to standard Fmoc-Gly-OH from other suppliers, the batch-to-batch consistency and thorough analytical documentation provided by baishixing offer distinct advantages. While many vendors cite similar purity levels, baishixing’s COA includes detailed impurity profiles and solvent residue data, enabling direct integration into GMP workflows. The company also maintains dedicated inventory for large-scale orders, reducing supply chain risk for ongoing peptide synthesis projects. For research groups and manufacturers who require reliable material with full traceability, this product is a practical choice.