Fmoc-Gly-OH (N-α-Fmoc-glycine) is a high-purity, Fmoc-protected amino acid derivative supplied for solid-phase peptide synthesis (SPPS), used primarily by research biochemists, process chemists, and contract manufacturing organizations to introduce glycine residues without racemization or side reactions.
| Parameter Item | Value | Supplementary Explanation |
|---|---|---|
| Product Name | Fmoc-Gly-OH | N-α-Fmoc-glycine, M.W. 297.31 g/mol |
| CAS Registry Number | 29022-11-3 | Reference standard for identity confirmation |
| Molecular Formula | C₁₇H₁₅NO₄ | C 68.68%, H 5.09%, N 4.71%, O 21.52% (theoretical) |
| Purity (HPLC) | ≥99.0% | Area normalization at 214 nm; lot-specific chromatogram included |
| Appearance | White to off-white crystalline powder | No lumps, free-flowing |
| Melting Range | 174–178°C | Determined by differential scanning calorimetry (DSC) |
| Specific Optical Rotation [α]²⁰D (c=1, DMF) | −5.0° to −6.0° | Measures enantiomeric excess of the whole molecule |
| Loss on Drying | ≤0.5% | After 3 h at 60°C under vacuum |
| Residual Solvents | ≤0.1% total (GC) | Meets ICH Q3C Class 3 limits |
| Heavy Metals | ≤20 ppm | ICP-OES analysis |
| Storage Conditions | 2–8°C, airtight, protected from light | Stability data based on ICH Q1A(R2) protocols |
| Shelf Life | 36 months from manufacturing date | Retest date printed on each label |
| Pack Size Options | 25 g, 100 g, 500 g, 1 kg | Bulk packaging (5 kg/10 kg) available on request |
1. What is the solubility of Fmoc-Gly-OH in DMF?At 25°C, Fmoc-Gly-OH dissolves completely in DMF at a concentration of ≥200 mg/L– actually, ≥200 mg/mL. A 0.5 M solution is readily prepared in less than 2 minutes with stirring, no heating needed.
2. How does Fmoc-Gly-OH compare to Boc-Gly-OH for SPPS?Fmoc-Gly-OH is preferred in Fmoc/tBu SPPS because its deprotection uses mild base (20% piperidine), unlike Boc chemistry requiring strong acid. This enables orthogonal synthesis of side-chain-protected peptides on acid-labile resins like Wang or Rink amide.
3. Can I use Fmoc-Gly-OH for peptide synthesis in aqueous solution?No. Fmoc-Gly-OH is poorly soluble in water (<1 mg/mL). Use organic solvents such as DMF, DCM, NMP, or dioxane. For aqueous coupling, consider water-soluble carbodiimide (EDC) with a co-solvent like DMF.
4. What are the main impurities monitored in this product?We routinely trace Fmoc-Gly-OH itself, N-protected glycine dimer, N-Fmoc-di-glycine, and residual piperidine adducts. Total impurities remain below 1.0% by HPLC, with a single max impurity ≤0.2%.
5. Does Fmoc-Gly-OH racemize during coupling?Glycine does not have a chiral center, so racemization is inherently not possible on the α-carbon. This is why glycine is often used as a control in racemization studies. However, epimerization of the C-terminal residue can still occur; a low water content (≤0.5%) helps minimize such events.
6. What storage conditions maintain the longest shelf life?Keep the original amber bottle tightly sealed at 2–8°C. Under these conditions—and away from light—the product remains stable for at least 36 months. Avoid repeated exposure to humidity; use a desiccator in humid climates.
7. Do you provide analytical data (CoA) with each batch?Yes. Every shipment includes a certificate of analysis containing HPLC purity, melting range, specific rotation, loss on drying, residual solvents, and heavy metal results. An HPLC chromatogram and the NMR spectrum are available can be provided via email upon request.
8. Is bulk packaging available for production use?We offer 1 kg, 5 kg, and 10 kg packaging in double-layer polyethylene bags inside a sealed fiber drum. Custom lot sizing is possible for long-term manufacturing contracts. All bulk lots are tested under a reduced sampling plan per ISO 2859-1.
Unlike many suppliers that offer Fmoc-Gly-OH with a simple minimum purity of 98% and no additional process data, baishixing provides a fully documented product with narrow specification ranges (purity ≥99.0%, free glycine ≤0.3%, D50 45–90 μm) and batch-level analytical traceability. The 36-month shelf-life guarantee backed by ICH stability studies reduces waste for both inventory managers and research groups, while the family of packing sizes—25 g to 10 kg—makes the same lot–to–lot consistency accessible to an academic laboratory and a commercial manufacturer alike. This Fmoc-Gly-OH is therefore best suited for researchers who require reproducible peptide assembly in a regulated environment or in a discovery setting where eliminating variables is critical.
