1. Product Overview
Triglycine (CAS 556-33-2) is a high-purity synthetic tripeptide biochemical reagent designed for biological conjugation, protein stability research and cryo-EM sample preparation, solving common industry pain points of low conjugation efficiency, uncontrollable protein conformational instability and excessive enzymatic side reactions.
2. Core Product Categories
Biochemical Research Reagent, Protein Stabilizing Additive, Enzymatic Conjugation Auxiliary Substrate
3. Target Crowd
Biomedical research institutions, biopharmaceutical R&D laboratories, structural biology teams, and enzymatic biomanufacturing enterprises
4. Core Solved Pain Points
· Traditional peptide substrates cause non-specific side reactions and low yield in enzymatic conjugation reactions
· Common protein protective agents induce background noise and interfere with microscopic imaging results
· Most auxiliary reagents fail to form stable hydrogen bond networks, leading to easy protein denaturation and activity loss
· Conventional linkers produce steric hindrance, restricting site-specific conjugation of single-chain antibodies
5. Core Selling Points
· With 99.0% minimum purity detected by HPLC, the product eliminates impurity interference and ensures repeatable experimental and production data.
· Zero side-chain reactive groups achieve 100% specific enzymatic recognition, completely avoiding non-specific cross-linking and invalid by-product generation.
· Standard molecular weight of 189.17 g/mol provides stable spatial flexibility, reducing steric hindrance and increasing antibody conjugation efficiency by 300% in enzymatic reactions.
· Ordered hydrogen bond network structure stabilizes protein conformation, reducing protein denaturation rate by 45% during cryo-EM sampling and low-temperature storage.
6. Core Product Parameters
This product features a fixed CAS number of 556-33-2 and a molecular formula of C6H11N3O4. It maintains a minimum HPLC purity of 99.0% and a molecular weight of 189.17 g/mol. The melting point ranges from 242°C to 244°C, with a water solubility of 100 g/L at 25°C. The product appears as white crystalline powder at room temperature, with a moisture content controlled below 0.5%. It is stored at 2°C to 8°C under sealed conditions with a 24-month shelf life to guarantee stable biochemical activity.
7. Application Scenarios
Structural biology researchers use this triglycine reagent for cryo-EM sample pre-treatment to reduce background noise and improve protein imaging definition.
Biopharmaceutical R&D personnel apply the product to single-chain antibody site-specific conjugation to improve reaction efficiency and reduce heterogeneous impurities.
Enzymatic biomanufacturing enterprises adopt the reagent as a reaction substrate to eliminate side reactions and lower overall production purification costs.
Protein engineering laboratories use the product as a protective additive to build hydrogen bond networks and lock protein biological activity for long-term preservation.
8. Frequently Asked Questions
Q1: What makes triglycine (556-33-2) avoid side reactions in enzymatic catalysis?
Triglycine has no reactive amino acid side chains, so it only binds target enzyme active sites, completely preventing non-specific cross-linking and auxiliary side reactions during catalysis.
Q2: How does triglycine improve the resolution of cryo-EM protein imaging?
It competes for air-water interface adsorption to reduce protein denaturation and aggregation, without generating background noise, effectively improving the clarity of microscopic particle imaging.
Q3: What storage conditions are required to maintain its biochemical stability?
Seal and store the product at 2°C to 8°C, avoid repeated freezing and thawing and direct strong light exposure, which ensures full performance within the 24-month shelf life.
Q4: Why can this product stabilize the biological activity of protein drugs?
It forms dense and ordered hydrogen bond networks around protein molecules, restricting polypeptide chain unfolding and resisting denaturation caused by temperature and freeze-thaw stress.
Q5: Is this triglycine suitable for antibody-drug conjugate research?
Yes, its high flexibility and zero-steric-hindrance feature support sortase-A mediated site-specific conjugation, improving ADC homogeneity and reaction yield effectively.
Q6: What is the solubility performance of this triglycine in experimental systems?
It achieves 100 g/L water solubility at 25°C, realizing uniform dispersion in aqueous biological systems without precipitation or stratification interference.
Compared with conventional peptide reagents and protein protective additives, Triglycine (CAS 556-33-2) features ultra-high biochemical specificity, zero side-reaction performance and unique flexible skeleton structure. It avoids the impurity interference, steric hindrance and unstable protection defects of similar products. It can simultaneously meet the technical demands of high-precision structural biology imaging, high-efficiency enzymatic conjugation and long-term protein stability maintenance, making it more suitable for professional biochemical research and high-standard biopharmaceutical laboratory application scenarios.
