High quality Fmoc-Gly-OH Factory quotation

Fmoc-Gly-OH (Fmoc-glycine, CAS 29022-11-3) is a N-α-Fmoc-protected glycine derivative designed for use in solid-phase and solution-phase peptide synthesis, providing peptide chemists with a high-purity building block that minimizes undesired by-products and improves reaction yields.

Core Selling Points

Purity ≥ 99% (HPLC) – Reduces accumulation of deletion peptides during chain elongation, leading to cleaner crude products and simpler purification steps.
Low piperidine-related impurities – Controlled levels of free amine (≤ 0.1%) and DMF-insoluble matter (≤ 0.05%) prevent premature deprotection or clogging in automated synthesizers.
Batch-to-batch consistency – Every lot is tested via HPLC, TLC, and MS; a Certificate of Analysis (CoA) is provided with the retention time, assay value, and impurity profile, enabling reproducible synthesis across experiments.
Solubility in standard coupling solvents – Fully dissolves in DMF, DCM, and NMP at concentrations up to 0.5 M at 25 °C, compatible with both microwave-assisted and room-temperature coupling protocols.
Stable under refrigerated storage – Retains > 99% purity for 24 months when stored at 2–8 °C in a sealed, desiccated container.

Detailed Specification Table

Parameter	Value	Supplementary Notes
Product Name	Fmoc-Gly-OH	N-(9-Fluorenylmethoxycarbonyl)glycine
CAS Number	29022-11-3	Unique chemical registry identifier
Molecular Formula	C₁₇H₁₅NO₄	Calculated average mass
Molecular Weight	297.31 g/mol	Exact mass: 297.100 g/mol
Appearance	White to off-white powder	Visual inspection under normal light
Purity (HPLC)	≥ 99.0%	Area percent, detection at 210 nm
Free Amine (TLC)	≤ 0.1%	After derivatization with ninhydrin
Loss on Drying	≤ 0.5%	3 h at 60 °C over P₂O₅
Melting Point	172–176 °C	Decomposition may occur; not a critical specification
Solubility (DMF)	≥ 100 mg/mL at 25 °C	Clear colorless solution
Storage Conditions	2–8 °C, tightly sealed	Protect from light and moisture; do not freeze
Packaging Options	25 g, 100 g, 500 g, 1 kg	Custom bulk packaging available

Applicable Scenarios

Solid-phase peptide synthesis (SPPS) – Researchers using Fmoc chemistry protocols rely on Fmoc-Gly-OH as the C-terminal or internal building block; the high purity reduces the need for repeated recrystallization of the final peptide.
Solution-phase fragment condensation – Medicinal chemists preparing short peptide fragments (2–10 residues) use this reagent in DCM or ethyl acetate with carbodiimide coupling agents, achieving near-quantitative yields without racemization.
Automated peptide synthesizers – Laboratories running high-throughput parallel synthesis benefit from its low particle content and consistent dissolution, preventing nozzle clogging and concentration variability.
Research scale-up studies – Process chemists evaluating coupling conditions or resin loading use Fmoc-Gly-OH to establish baseline kinetics, owing to its stable impurity profile.
Clinical-quality peptide production – GMP-oriented facilities that require extensive documentation use this product's COA and HPLC data to support batch release documentation.

Frequently Asked Questions

What is the CAS number of Fmoc-Gly-OH?
The CAS number is 29022-11-3. This identifier is used across chemical databases and supplier catalogs for unambiguous ordering.

How should Fmoc-Gly-OH be stored in the laboratory?
Store at 2–8 °C in the original tightly closed container. Keep away from strong bases, oxidizing agents, and direct light to prevent Fmoc cleavage or discoloration.

Can Fmoc-Gly-OH be used in microwave-assisted synthesis?
Yes. It is stable under standard microwave SPPS conditions at 60–80 °C for 5–10 minutes per coupling. Always use pre-cooled or fresh solutions for optimal performance.

What is the typical coupling protocol for this reagent?
Dissolve 3 equivalents of Fmoc-Gly-OH in DMF, activate with HBTU/HOBt or DIC, and react with resin-bound amino groups for 30–60 minutes at room temperature. Double coupling is recommended for difficult sequences.

How is purity determined for Fmoc-Gly-OH?
Purity is measured using reverse-phase HPLC at 210 nm, with the main peak area normalized to 100%. Confirmation is performed by electrospray mass spectrometry (ESI-MS) and thin-layer chromatography.

Does this product contain any free glycine impurities?
Free glycine content is kept below 0.1% (by HPLC after derivatization). This low level prevents unwanted chain termination at the N-terminus during SPPS.

Can Fmoc-Gly-OH be used for peptide modifications other than linear synthesis?
Yes. It can be incorporated into peptide backbones, used for native chemical ligation after conversion to a thioester, or paired with C-terminal amide resin to prepare glycine-rich peptides.

What are the main differences between Fmoc-Gly-OH and Boc-Gly-OH?
Fmoc-Gly-OH is cleaved with piperidine under mild basic conditions, suitable for acid-sensitive side-chain protection. Boc-Gly-OH requires strong acid deprotection (TFA), which is incompatible with many base-labile linkers.

Differentiation Summary

Compared to standard commercial Fmoc-glycine, the baishixing grade Fmoc-Gly-OH offers a strictly controlled impurity profile, a documented free amine content of ≤ 0.1%, and long-term stability data that are not uniformly provided by competing suppliers. This makes it better suited for automated synthesis, scale-up studies, and any workflow requiring reproducible yields across batches. For laboratories needing a cost-effective, high-purity glycine building block without restoring to ultra-expensive GMP material, this product provides a balanced solution. Choose it when you prioritize consistency, documented quality, and reliable technical support over brand-name premium pricing.