Fmoc-Gly-OH 29022-11-5 Manufacturer

Product Definition

Fmoc-Gly-OH (N-(9-Fluorenylmethoxycarbonyl)glycine, CAS 29022-11-3) is a purified amino acid derivative for peptide chemists, biopharmaceutical researchers, and CRO laboratories that require ≥98% HPLC purity and consistent coupling performance in Fmoc solid-phase peptide synthesis.

Core Selling Points

Batch purity ≥98% (HPLC): Delivers predictable coupling efficiency in each cycle, minimizing deletion sequences and reducing final purification time for target peptides.
Documented physical identity: Molecular weight 297.31 g/mol and melting range 172–176 °C (decomp.) provide verifiable constants for batch confirmation and GMP record-keeping.
Controlled residual solvents: Headspace GC analysis per lot confirms low levels of process solvents, preventing unpredictable side reactions during activation with HBTU, HATU, or DIC reagents.
Transparent lot documentation: Each shipment includes a COA reporting HPLC purity, melting range, water content (≤1.0% by Karl Fischer), and appearance, enabling research audits and scale-up reviews.
Stable under recommended storage: A 24-month shelf life at 2–8 °C in light-protected, airtight packaging reduces batch-to-batch inconsistency in multi-year research programs.

Detailed Parameter Table

Parameter	Value	Supplementary Notes
Product Name	Fmoc-Gly-OH	N-(9-Fluorenylmethoxycarbonyl)glycine
CAS Number	29022-11-3	—
Molecular Formula	C₁₇H₁₅NO₄	—
Molecular Weight	297.31 g/mol	Calculated from standard atomic weights
Purity (HPLC)	≥98.0%	Area normalization method
Appearance	White to off-white crystalline powder	Visual inspection
Melting Point	172–176 °C (decomp.)	Capillary method
Water Content	≤1.0%	Karl Fischer titration
Solubility	Soluble in DMF, DMSO; sparingly soluble in dichloromethane	Visual dissolution test
Storage Condition	2–8 °C, airtight, light-protected	24-month shelf life
Package Sizes	25 g, 100 g, 500 g, 1 kg	Bulk quantities available on request

Application Scenarios

Automated and manual SPPS: Peptide synthesis laboratories use Fmoc-Gly-OH for N-terminal or internal glycine positions, with defined purity supporting reproducible cycle-to-cycle coupling.
Peptide API development: Pharmaceutical process chemists use this building block in route scouting and process validation, relying on the documented impurity profile for technology transfer.
Custom peptide manufacturing: CRO/CMO facilities producing research-grade or GMP-grade peptides select this glycine derivative to maintain batch consistency within customer timelines.
Peptide-drug conjugate research: Bioconjugation teams synthesize linker and payload constructs with Fmoc-Gly-OH, benefiting from the orthogonal Fmoc protection for site-specific reactions.
Analytical reference standards: Quality control chemists use the stable melting range and defined HPLC response to validate methods and calibrate instruments for amino acid analysis.

Frequently Asked Questions

What is the CAS number of Fmoc-Gly-OH?Fmoc-Gly-OH is registered under CAS 29022-11-3. Verify this identifier against the supplied COA and SDS when receiving the material, as cross-referencing the CAS registry number with the batch certificate prevents mix-ups with other Fmoc-protected amino acids and supports correct documentation in your inventory records.

Is Fmoc-Gly-OH suitable for microwave-assisted SPPS?Yes. The Fmoc group tolerates the standard microwave SPPS temperature range of 70–90 °C without premature deprotection. Use the same coupling times and activator concentrations as for room-temperature protocols; no equipment adjustments or special cooling steps are necessary.

How should I store Fmoc-Gly-OH to maintain purity?Store at 2–8 °C in an airtight container, protected from light and moisture. Keep the desiccant pouch inside the bottle, allow the container to equilibrate to room temperature before opening, and reseal immediately after sampling to avoid hygroscopic damage.

What is the typical purity specification for this product?Batch purity is determined by HPLC and reported as ≥98.0% area under the main peak. Each shipment includes a COA stating actual purity, water content, and melting range, with lot-specific data retained for research records and internal audits.

Can Fmoc-Gly-OH be used in large-scale peptide manufacturing?Yes. Packaging up to 1 kg per container is available for scale-up campaigns. Consistent HPLC purity and controlled residual solvent levels across lots support direct technology transfer from research scale to pilot or production reactors without re-bracketing experiments.

Does Fmoc-Gly-OH racemize during coupling?No. Glycine lacks a chiral α-carbon, so racemization is structurally impossible. This distinguishes it from other Fmoc-amino acids, eliminates a common quality variable, and makes coupling conditions easier to optimize during sequence design.

What solvents can dissolve Fmoc-Gly-OH?It dissolves completely in DMF and DMSO at working concentrations. For dichloromethane-based coupling systems, dissolve the powder first in a minimal volume of DMF (10–20% of total reaction volume) before dilution, ensuring complete activation for the coupling step.

What is the melting point reported on the COA?The melting point range is 172–176 °C with decomposition, measured by capillary method and reported per lot on the COA. This range serves as a quick identity check and differs clearly from other Fmoc-amino acids.

Differentiation Summary

Compared to generic glycine derivatives with less defined impurity profiles, Fmoc-Gly-OH from baishixing combines ≥98% HPLC purity, the documented melting range of 172–176 °C, and a complete COA delivered with every batch. The absence of a chiral center in glycine removes racemization risk, which makes this product the appropriate choice for laboratories that prioritize reproducible coupling efficiency and traceable documentation. It is best suited for peptide synthesis teams, CRO analytical groups, and process development scientists who need a well-characterized Fmoc-glycine building block from research quantities through bulk supply.

Data references: Physicochemical constants (CAS number, molecular formula, molecular weight, melting range) are consistent with values published in the CAS Registry, the Merck Index, and the per-batch Certificate of Analysis issued by baishixing.