Fmoc-Gly-OH (Fmoc-glycine) is a high-purity, N-protected glycine derivative used in solid-phase and solution-phase peptide synthesis, providing chemists and biopharmaceutical manufacturers with a reliable building block that minimizes racemization and ensures consistent coupling efficiency.
| Parameter | Value | Supplementary Notes |
|---|---|---|
| Product name | Fmoc-Gly-OH | Also known as Fmoc-glycine |
| CAS number | 29022-11-3 | Standard registry number |
| Molecular formula | C₁₇H₁₅NO₄ | Molecular weight: 297.31 |
| Assay (HPLC) | ≥99.0% | Area normalization method |
| Enantiomeric purity | ≥99.9% (ee) | Determined by GC |
| Appearance | White to off-white powder | Visual inspection |
| Water content (KF) | ≤0.5% | Karl Fischer titration |
| Residual solvents | ≤0.3% | According to USP<467> |
| Solubility | Soluble in DMF, DMSO, NMP | ≥100 mg/mL in DMF at 25 °C |
| Storage conditions | 2–8 °C, sealed, dry, light-protected | Shelf life: 24 months |
| Packaging | 25 g, 100 g, 1 kg, 5 kg, 25 kg | Custom packaging available on request |
What is the solubility of Fmoc-Gly-OH in DCM?
Fmoc-Gly-OH is only partially soluble in dichloromethane (about 5 mg/mL). For stock concentrations above 100 mg/mL, use DMF, DMSO, or NMP.
How should Fmoc-Gly-OH be stored?
Store in a tightly sealed amber glass container at 2–8 °C, kept dry and away from light. Under these conditions, purity remains ≥99% for at least 24 months.
Is Fmoc-Gly-OH compatible with automated peptide synthesizers?
Yes. Its defined particle size (200–400 mesh) promotes accurate weighing and rapid dissolution, and it behaves well in standard HBTU/HOBt coupling cycles.
What coupler works best with Fmoc-Gly-OH?
HBTU or HATU with DIPEA in DMF is recommended. Using 4 equivalents of amino acid, 4 equivalents of HBTU, and 8 equivalents of DIPEA gives >95% coupling within 30 minutes at room temperature.
Can I obtain a certificate of analysis (CoA) before purchase?
Yes. A CoA that includes HPLC purity, water content, residual solvents, and heavy metal analysis is provided with every shipment. Batch-specific data is available upon request.
Does Fmoc-Gly-OH require special safety handling?
Standard laboratory precautions apply: use a fume hood, wear nitrile gloves and safety glasses, and avoid dust inhalation. It is not classified as dangerous goods for transport (non-GHS hazardous).
What is the typical deprotection time for the Fmoc group?
Treat the resin with 20% piperidine in DMF for 5 minutes, then repeat for 10 minutes. This removes the Fmoc group quantitatively without affecting the glycine side chain.
Compared with other commercial sources, Baishixing's Fmoc-Gly-OH delivers a combination of tight impurity control (single impurity ≤0.1%, residual solvents ≤0.3%) and a consistent physical form that reduces dissolution time in automated workflows. Each batch is documented with full analytical data, allowing process development teams to validate resin loading and coupling results without re-testing raw materials. For researchers and producers who need reliable batch-to-batch reproducibility, transparent documentation, and cost-effective bulk supply, this Fmoc-Gly-OH provides a practical option over generic fine-chemical grades.
