N6-Cbz-L-lysine has demonstrated excellent biocompatibility in tissue engineering, with relevant research as follows:
In one study, N6-Cbz-L-lysine was combined with γ-benzyl-L-glutamate to synthesize a bifunctional copolymer peptide polymer, which was then fabricated into a fibrous scaffold via electrospinning for application in neural tissue engineering. Experiments confirmed that this copolymer peptide scaffold exhibits good biocompatibility and no cytotoxicity to nerve cells. It can promote the adhesion of nerve cells, allowing PC-12 neural cells to grow and differentiate well on it without the need for cell adhesion coatings, unlike on ordinary cell culture plates. Compared to the single-component polypeptide scaffold in the control group and polystyrene cell culture dishes, PC-12 cells grown on this copolymer peptide scaffold showed higher neuronal activity and longer neurite lengths.
Further, after partially hydrolyzing the copolymer peptide into a positively charged scaffold, its hydrophilicity, cell affinity, and recognizability were enhanced. When PC-12 cells grew on this positively charged scaffold, their cell activity reached the optimal level, and the neurite length increased by 32% compared to the control group, reaching 174μm. In terms of cell differentiation, the PC-12 cells cultured on this fibrous scaffold showed a higher degree of differentiation. The expression of relevant neural differentiation markers, GAP43 and Synapsin1, increased significantly under the treatment of nerve growth factor (NGF), indicating that the copolymer peptide fibrous scaffold synthesized with N6-Cbz-L-lysine not only has good biocompatibility but also can synergize with NGF to promote the differentiation of nerve cells.
N6-Cbz-L-lysine has good biocompatibility in tissue engineering. Materials prepared based on it have great application potential in the field of tissue engineering, especially in neural tissue engineering, as they can provide a favorable growth environment for cells and promote cell adhesion, growth, and differentiation.
